Unknown

yard Negative Unknown Upon being given a bacteriuml elaboration containing two bacterium, I was assigned the task of set a bust the several(prenominal) bacteria. The two bacteria of the mixture were 1 one thousandme collateral and one gigabyte negative. The experiment has two split and this is the part of isolating the gigabyte-negative bacteria. The technique used was called streaking for closing give rise through in which the bacteria were streaked thinly on food for thought agar in different directions in a normal that left several(prenominal) colonies on the last growth streak. These individual colonies could be identified by their individual characteristics such as color and shape and distinguished from one another. This was not tardily with this ending because both(prenominal) bacteria grew in colonies that were small, white, and shaped similar. To sieve these bacteria I had to first gram stain the bacterial mixture to see if I had both types of bacte ria and so I used the following travel to perform the gram stain. 1. The bacteria are discolourationed on a slide and allowed to modify. 2. The bacteria is heat fixed to the slide 3. lechatelierite violet pigment is added to the smear for 1 minute 4. purify the pigment complete with distilled peeing 5. Apply Grams Iodine for 1 minute 6. Wash iodine off with distilled water 7.

Decolorize the smear with 95% ethyl souse beverage hurtle by drop until it runs off discharge 8. Wash off alcohol with distilled water 9. Counterstain with Safranin for 45 seconds 10. Wash off Safranin with distilled water 11. Blot dry with Bibulous paper Foll! owing this test, I found that I had a lot more gram negative bacteria than the gram positive and so I could easily move one colony of my streaked purification and have a high chance of growing a pure culture from one of the isolated colonies. So, with this method, I grew a pure culture of the gram negative bacteria that I would use in my tests to determine its identity. I ran several tests on the bacteria unadulterated with the Gram stain that proved that the bacteria were gram negative....If you wish to get a full essay, couch it on our website: OrderCustomPaper.com

If you want to get a full essay, visit our page: write my paper